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Types of Specimen Collection Containers

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Types of Specimen Collection Containers

Blood Collection Containers – Common Vacutainer Tubes

ORDER OF DRAW	CAP COLOUR	ADDITIVE	BLOOD VOLUME	SUITABLE FOR / PURPOSE	NO. OF INVERSIONS AFTER COLLECTION
1		Culture	10mL	Bacteriology and fungal studies	8
2		Sodium Citrate	2.7mL	Coagulation studies. The ratio of blood to anticoagulant is critical for valid prothrombin time and activated partial thromboplastin time results. Note: Fill to mark to ensure correct blood to additive ratio.	4
3		ACD Tube	6mL	HLA, tissue typing, DNA studies	8
4		Streck (Cell Free DNA)	10mL	Cell free Plasma DNA	10
5		Clot Activator (Plain) Paediatric tube	6mL 0.5mL	Most chemistry, including drug levels and serological tests that require serum. Also for immunohaematological tests that require clotted blood such as abnormal blood group antibody tests and crossmatching requests.	5
6		SST II (Gel)	5mL	Chemistry, Immunology and Serology studies. All tests requiring serum except those few that need red cells as well (such as abnormal blood group antibody screen, cold agglutinins).	5
7		Trace Element Serum	6mL	Trace Elements/ Toxicology	8
8		Lithium Heparin	4mL	Tests requiring plasma or whole blood (such as cytogenetics when using blood DNA) and STAT biochemistry tests such as electrolytes, renal screen, ammonia test.	8
10		EDTA (GXM)	6mL	For cross-matching requests only.	8
11		EDTA Paediatric tube	3mL 0.5mL	Most haematological tests, HbA1C, Cyclosporine, Homocysteine, and molecular genetics tests (when using blood DNA).	8
12		Trace Element (K2EDTA)	6mL	Trace Elements/ Toxicology	8
13		Potassium Oxalate	2mL	Chemistry (Glucose)	8

Note: Failure to fulfill above requirements may result in specimen rejection.

Other Specimen Collection Containers

TYPE OF CONTAINERS/DESCRIPTION	COMMENTS
Double eSwab (flocked swabs with Amies media) Pink eSwab - Sample axilla and groin, swirl in Amies media and discard swab White eSwab - Sample the anterior nares and place it in the tube of Amies media	For MRSA PCR Screening. Please refer to the instructions on page 23
CULTURE SWAB with Amies Transport Media Sterile viscose swab attached to plastic applicator stick with transport media	For bacteriological culture from all sites. It contains a special transport media which will maintain micro-organism viability but does not promote growth of micro-organisms. Blue-cap swab is suitable for both aerobic & anaerobic organisms.
DRY SWAB without Transport Media Sterile cotton swab attached to plastic applicator stick	For Chlamydia Trachomatis PCR test.
STOOL CONTAINER Screw cap (brown) plastic container with attached spatula	For collecting stool specimen for routine examination and culture.
URINE CONTAINER, Sterile (UNIVERSAL CONTAINER) Sterile plastic container with screw cap	For routine urinalysis and urine culture. Also suitable for collecting body fluids and sputum for routine examination or culture.

For Genetics Department

TYPE OF SUPPLIES	COMMENTS
BOTTLE OF TRANSPORT MEDIUM	For QF-PCR and Karyotyping on Chorionic Villus or Products of Conception
BOTTLE OF TRANSPORT MEDIUM	For Bone Marrow Karyotyping
MOTTLED BLACK/TAN Cell-Free DNA BCT has chemical additives 10mL x 2	For non-invasive fetal aneuploidy screening (NIPS) tests using maternal blood and circulating tumour DNA testing in oncology. Avoid possible backflow from the tube. Tube contents should not touch stopper or the end of the needle during the collection procedure. Order of Draw: Perform Cell-Free DNA BCT tubes first. After the tube is filled completely, gently invert 8 to 10 times.
PLAIN STERILE UNIVERSAL BOTTLE	For Amniotic Fluid Karyotyping or QF-PCR
SODIUM OR LITHIUM HEPARIN BLOOD TUBE	For Karyotyping and FISH
EDTA BLOOD TUBE	For majority of Molecular Genetics tests

Miscellaneous Supplies

TYPE OF SUPPLIES/DESCRIPTION	COMMENTS
CAPILLARY TUBE (Heparinised) Self sealing	For neonatal serum bilirubin test. Collect 2 or 3 capillary tubes (heparinised) of blood.
CONTAINER, with Formalin Clean container (50mL) half-filled with formalin	Supplied for transporting small histopathology specimens.
GLASS SLIDE Rectangular 3"x1" glass slide with frosted end	Supplied for collecting pap smear and cytology smears only. Please write patient's name on the frosted end of slide in pencil.
SLIDE HOLDER (Pap Smear Container) Blue or white plastic holder for 2 slide wells. Supplied with 95% alcohol for fixation of smears.	Supplied for holding cytology and pap smear glass slides only.
SPATULA (Scraper) Wooden stick with paddle	Use to collect material for cytology smears.
SYRINGES and NEEDLES Disposable syringes & needles	For collecting blood into non-vacutainer tubes, i.e. conventional screw-top containers. Sizes available for syringes: 20mL, 10mL, 5mL & 2.5mL Sizes available for needles: 23G 1", 22G 1½", 21G 1½"
VACUTAINER NEEDLE Green 21G vacutainer needle, regular size	Disposable vacutainer needle to be used together with vacutainer needle holder for drawing blood specimen into vacutainer tubes.
DISPOSABLE VACUTAINER NEEDLE HOLDER White hollow plastic tube	Used to hold vacutainer needle and the vacutainer tube while collecting blood specimen.
OTHER SUPPLIES, include non-vacutainer collection tubes, thin prep vial with cytobrush etc.	Please call 6278 9188 to request for other supplies.

Blood Draw Yellow Top Tube

Source: https://www.parkwaylabs.com.sg/resources/specimen-collection-and-handling/supplies/specimen-collection-handling/types-of-specimen-collection-containers

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